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  • 德国MoBiTec公司于1987年由StephanDiekmann教授创立,由于和Max-Planck协会(德国处于领先地位的研究中心)有着密切的关系,这使得本公司与很多具有创造性的科学家有着亲密的合作。另外,MoBiTec拥有来自欧洲和北美的研究人员,包括法国的Pasteur研究中心和荷兰的Vrije大学的科学家。MoBiTec公司能提供独一无二的高质量产品,从而简化实验设计,而留出更多更宝贵的时间用于实际的研究。
    产品包括:双杂交和单杂交系统——体内蛋白-蛋白和DNA-蛋白关系,噬菌体展示系统——筛选特殊的多肽配体和选择具有特异性结合特性的新的蛋白质,LamdaPS基因文库,用于克隆的多功能DNA载体,靶基因的表达与分析,重组蛋白质高效表达和纯化试剂盒细胞转染试剂和细胞培养工具,各种固体和溶解状态的酶,基因组学和蛋白组学研究产品(PCR,核酸,蛋白质纯化分析),各种抗体、细胞因子、生长因子、重组蛋白质,高级荧光试剂和试剂盒(蛋白质标记试剂盒,标记抗体,细胞染色试剂,钙指示器),亲和层析仪,凝胶分析工具——凝胶柱和凝胶电泳附件。
    BacillusmegateriumExpressionSystem
    BacillussubtilisExpressionVectors
    pBacTagTaggingVectors
    pPICHOLIShuttleVectorSystem
    Broad-Host-RangeVectorspBBR122&pBHR1
    FusionProteinCloningSystemPheBo
    FusionProteinCloningSystempAX
    BRPPlasmidandCompetentBRPCells
    pPEPTIDECloningVector
    Promoter-TrapVectorpBBRRESO
    pORF-CLONEVectorSystem
    ExontrapCloningVector
    PCRCloningVectorp3T
    SuicideVectorpCorrectClone
    ssDNA-ProductionandExpressioninpMEX
    His-tagCloningVectorpEG-His1
    MultipleCloningSiteVectorpMCS5

    YourpartnerforuniqueTwo-andOne-Hybrid-Systems,PhagemidDisplay,GenomicLibraries,DNAVectorSystems,CellBiologyandAntibodies,DNAandProteinTools,FluorescenceTechnology,AffinityChromatography,andLabSupplies


    aboutMoBiTec
    History

    nnovativeToolsforMolecular&CellBiology

    MoBiTecGmbHisaprivatelyheldcompanythatwasfoundedbyProf.Dr.StephanDiekmannin1987.ThecloserelationtotheMax-PlanckSocietyaswellasthelocationinGoettingen,oneofGermany’sleadingresearchcenters,facilitatedclosecooperationwithsomeofGermany’smostinventivescientists.Inaddition,MoBiTechasalwaysbeenoperatingextensivelywithscientistsalloverEuropeandNorthAmerica,includingthosefromthePasteurInstituteinFranceandVrijeUniversityintheNetherlands.Bycommercializingthebrilliantideasofmanyresearchers,MoBiTecisabletoofferauniquerangeofhighqualityproductswithsimplifiedprotocolsmakinglablifeeasierandleavingmoretimeforactualresearch.

    Productsincludee.g.two-&onehybridsystemsforinvivodetectionofprotein-proteinorDNA-proteininteractions,aphagemiddisplaysystemforscreeningspecificpeptideligandsandselectingnovelproteinswithspecificbindingproperties,convenientLambdaPSgenomiclibraries;versatileDNAvectorsforcloning,expressingandanalyzingtargetgenes,kitsforefficientexpressionandpurificationofrecombinantproteins,celltransfectionreagentsandcellculturetools,immobilizedandsolubleenzymes,manyproductsforgenomicsandproteomicsresearch(e.g.forPCR,nucleicacid&proteinpurificationandanalysis),numerousantibodies,cytokines,growthfactorsandrecombinantproteins,superiorfluorescencereagentsandkits(e.g.proteinlabelingkits,labeledantibodies,cellstainingreagents,calciumindicators),affinitychromatographyproducts,gelanalysistoolsaswellasgenerallaboratoryequipmentsuchaslabcolumnsandgelelectrophoresisaccessories.

    Inparalleltoitsownproductlines,MoBiTecdistributesproductsfromseveralinternationalcompaniesinGermanyincludingfluorescentprobesandresearchchemicals(MolecularProbes,AnaSpec,Teflabs),quantumdots(EvidentTechnologies),avarietyofDNApurificationkitsandDNApolymerases(EdgeBiosystems,TaKaRa),diagnostictools(Biomerica,Cytocell),gelelecrophoresisaccessories(Amresco),glycobiologyproducts(DextraLaboratories),high-qualitybiochipproducts(GenTel),celltransfectionreagents(Mirus)aswellasantibodies,proteins,substrates,finechemicalsandkitsformolecularandcellbiology-inparticularvirology,immunology,neurobiology,apoptosis,signaltransduction,cellproliferation,cytotoxicityandcancerresearch(MBL,A.G.Scientific,TaKaRa,Amresco,ViroGen,AnaSpec).

    MoBiTecoffersatotalservice,beginningwiththeclearandfulldocumentationofproductsextendingtoindividualadviceandtechnicalserviceforcustomers.MoBiTecproductsaredistributedworldwide-inGermanyfromourhomeoffice,inothercountriesbydistributors.Moreover,MoBiTeciscontinuouslylookingfornewproductsandmarketabletechnologiesforlicensingandisthereforestronglyinterestedinscientificcollaborationsinordertodevelopinnovativeproductideas.


    • 药准字:
    • 主要组成成分:mobitec的巨大芽孢杆菌表达系统
    • 性状:mobitec的巨大芽孢杆菌表达系统
    • 适应症:mobitec的巨大芽孢杆菌表达系统
    • 规格:mobitec的巨大芽孢杆菌表达系统
    • 贮藏:mobitec的巨大芽孢杆菌表达系统
    • 有效期:36
    • 生产企业:德国

    hp-vector表达系统megaterium芽孢杆菌
    hp-vectorexpressionsystemsforbacillusmegaterium
    features:
    highperformancevectorswithoptimizedsequence
    proteinyieldupto10timesenhancedcomparedtoproteinexpressionwithbasicplasmid
    plasmidswithestablishedmcs
    encodingc-orn-terminalhis-tagforversatilepurification(native,6xhis-tag)
    secretionwithlipaoryochsignalpeptideupto9foldincreased
    optimizedhighperformance(hp)vectorsforbacillusmegaterium!
    mobitecprovidesawiderangeofusefulvectorsfortheb.megateriumsystemthatareadaptabletomostapplicationsandproteinpurificationmethods.theseincludevectorscarryingasecretionsignalpeptidesequenceanda6xhis-tag.
    withournewhp-vectorsyieldsupto10-foldhighercomparedtothebasicplasmidsarepossible.allplasmidshaveestablishedmultiplecloningsites(mcs)forversatilecloning.furthermoreweoffervectorsencodingc-orn-terminalhis-tagsforeasypurification.theproteinsecretionwithlipaoryochsignalpeptidesisuptonine-foldincreased.inductionofproteinexpressionofallvectorsisachievedbythetightlyregulatedandefficientlyinduciblexyloseoperon.
    versatilesystemwithawiderangeofvectors
    theb.megateriumexpressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmall-andlarge-scale.
    b.megateriumhasproventobeanexcellentalternativehosttoe.coliforheterologousgeneexpression.unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.inaddition,therearenoendotoxinsfoundinthecellwall.
    b.megateriumprotoplastsspecificallyoptimizedfortransformation
    theprotoplastssuppliedbymobitecarefromb.megateriumstrainwh320,developedbyprof.dr.w.hillenattheinstituteofmicrobiologyinerlangen,germany.theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.
    forsecretionvectorsweofferprotoplastsofstrainms941.bothstrainsaremutantsofdsm319,wherewh320isachemicallymutant,whilems941hasadefineddeletioninthegeneofthemajorextracellularproteasenprm.
    orderinformation,shippingandstorage
    pleaseclickontherespectiveorder#forfurtherdetails.
    order#descriptionamount
    bmeg02bacillusmegateriumprotoplastsreadyfortransformation(strainwh320;1)
    materialissufficientfor4transformationspluscontrolexperiment.5x500µl
    bmeg50bacillusmegateriumprotoplasts,strainms9415x500µl
    bmeg31pc-his1623hpshuttlevector,c-term.6xhis-tag;lyophilizeddna10μg
    bmeg32pn-his-tev1623hpshuttlevector,n-term.his-tag,tev-site10μg
    bmeg30p3stop1623hp,shuttlevector,lyophilizeddna10μg
    bmeg33psplipa-hp,shuttlevectorwithsignalsequence,lypophilizeddna10μg
    bmeg34pspyoch-hp,shuttlevectorwithsignalsequence,lypophilizeddna10µg
    shippedatrt,protoplastsshippedondryice
    storelyophilizedvectorsat4°c,reconstitutedvectorsat-20°c,protoplastsat-70°c
    allvectorsaree.coli/b.megateriumshuttlevectors
    pleasenotethatprotoplastsofbacillusmegateriumhavealimitedshelflifeofsomemonthsonly,dependingonthedateofproduction.thereforepurchaseanduseofprotoplastsmustbethoroughlyplanned.

    标准即用型硒代蛋氨酸培养基

    基于合成的M9基本培养基,补充了葡萄糖,维生素和氨基酸(L-蛋氨酸除外)。

    该即用型和省时产品仅可从MolecularDimensions获得。

    细菌生长可以在L-蛋氨酸或L-硒代蛋氨酸(SeMet)的存在下进行。

    质粒表达构建体应转化为需要营养缺陷的蛋氨酸。

    Novagen的OvernightExpress™自动诱导系统I可以使用无葡萄糖选件。为什么不尝试使用新型Dream过夜表达培养基呢?

    \"它易于使用,我很高兴它包含在一个完整的套件中。”

    朱莉娅·格林菲尔德(JuliaGreenfield),美国马里兰大学

    产品编号产品名称单位大小单价数量

    MD12-500硒代蛋氨酸中等完成1套MOLECULARDIMENSIONS1

    MD12-501硒代蛋氨酸培养基和营养混合物620克MOLECULARDIMENSIONS1

    MD12-501B硒代蛋氨酸培养基500克MOLECULARDIMENSIONS1

    MD12-502硒代蛋氨酸中等营养混合物120克MOLECULARDIMENSIONS1

    MD12-503硒代蛋氨酸溶液(250x)16毫升MOLECULARDIMENSIONS1

    MD12-503B硒代蛋氨酸溶液(250x)50毫升MOLECULARDIMENSIONS1

    微批量实验的目的是提高晶体的尺寸和质量,这些晶体是由小的无用的晶体,孪晶或沉淀物形成的阵雨而获得的。正因为如此,微批量实验使用油障的插入来减慢蒸气扩散实验。

    使用Microbatch方法需要在滴液和储液器上放置一层油,并且可以通过使用不同的油(石蜡或有机硅)或两种油的不同比例的混合物来控制蒸发速率。微批料结晶试验通常在石蜡油,硅油或两者的混合物下进行(D\'Arcy等人,1996),因为已发现石蜡和硅油是惰性的,不能与水混溶。

    无容器结晶消除了结晶试验及其支撑容器之间表面接触的影响。

    该技术的基本原理是将结晶滴分配到一个容器中,在该容器中,一层低密度油被置于一层高密度油之上。这两个层是不可混溶的,并且在两种油的密度之间具有密度值的结晶滴将漂浮在界面上,因此不会接触容器壁。


    苏州蚂蚁淘生物科技有限公司SuZhouebiomallBIOLOGicalTechnologyCo.,Ltd.成立于2017年,经过数年的努力已迅速成为集科研和生产为一体的专业化生物工程公司。特别是ELISA研发,代测,技术服务这一产品已使上海抚生成为中国知名公司。公司产品涵盖ELISA研发、细胞培养,各种生化试剂、各种酶类、分子生物学试剂盒、培养基、自产实验室耗材、小型仪器等。此外、上海抚生还代理了美国药典标准品,中检所标准品,sigma,ScienCell,ATCC,wak0,omega,Worthington、MBI、Bioworld、Calendon等国外各类知名公司的产品。上海抚生将进一步加强人才经营、产品经营,不断提升企业的核心竟争力,实现具有高科技产业体系、知识化创业团队的国际化的公司,服务于生命科学领域,迎接世界经济一体化所带来的机遇与挑战。